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MIC values of ceftriaxone, <t>cefixime,</t> and penicillin G in FA19 strains transformed with penA19 containing the specified mutations from penA41 (forward; A ) or reversion of these mutations in penA41 back to their counterparts in penA19 (reversion; B ). The bars represent the median MICs with individual replicates shown as points (n = 3).
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MIC values of ceftriaxone, cefixime, and penicillin G in FA19 strains transformed with penA19 containing the specified mutations from penA41 (forward; A ) or reversion of these mutations in penA41 back to their counterparts in penA19 (reversion; B ). The bars represent the median MICs with individual replicates shown as points (n = 3).

Journal: PLOS Pathogens

Article Title: Antibiotic-resistance mutations in penicillin-binding protein 2 from the ceftriaxone-resistant Neisseria gonorrhoeae strain H041 strike a delicate balance between increasing resistance and maintaining transpeptidase activity

doi: 10.1371/journal.ppat.1013721

Figure Lengend Snippet: MIC values of ceftriaxone, cefixime, and penicillin G in FA19 strains transformed with penA19 containing the specified mutations from penA41 (forward; A ) or reversion of these mutations in penA41 back to their counterparts in penA19 (reversion; B ). The bars represent the median MICs with individual replicates shown as points (n = 3).

Article Snippet: Ceftriaxone sodium salt hemiheptahydrate and cefixime trihydrate were purchased from Thermo Fisher.

Techniques: Transformation Assay